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| Registros recuperados: 18 | |
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| Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne. |
| Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!. |
| Tipo: Text |
Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf |
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| Bachere, Evelyne; Chagot, Dominique; Grizel, Henri. |
| There is an ongoing need to isolate and purify ceIls in bivalve mollusc disease research. Many separation techniques differentiate cells solely by a single factor and usually require additives in the medium that may damage live cells. Centrifugai elutriation is a technique that separates particles by size and density simultaneously and can be conducted in physiological media without additives. Living cells can be separated without chemical damage and, because the procedure can be conducted aseptically, cells can be maintained in vitro after elutriation. A description of the technique, methods to determine flow rates and rotor speeds, and a preliminary separation of oyster hyalinocytes is presented |
| Tipo: Text |
Palavras-chave: Bivalve mollusc; Centrifugal elutriation; Cell separation. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/1988/publication-3118.pdf |
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| Gerard, Andre; Peignon, Jean-marie; Chagot, Dominique. |
| Individual determination of ploidy level in experimentally induced triploid bivalve molluscs using image cytometry. The sexual maturation in bivalve molluscs causes decreased growth and sometimes increased mortalities. These drawbacks can be avoided by genetic sterilization (triploidy). Triploïdy induction require a egg treatment and a ploidy control at each generation. The ploidy level is usually determined by counting the chromosomes, a technique which is relatively easy for the trochophora stage and for juveniles measuring from 5 mm to 3 cm only. ln order to be able to follow up the evolution of the ploidy rate during any stage of animallife, we have developed an estimation method based on image cytometry. Individual DNA content is determined by... |
| Tipo: Text |
Palavras-chave: Image cytometry; Caryology; Triploidy; Bivalve; Imagerie numérique; Caryologie; Triploïdie; Bivalve. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-3202.pdf |
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| Bodoy, Alain; Garnier, Jacqueline; Heurtebise, Serge; Nollet, V.; Chagot, Dominique. |
| La croissance et la reproduction du pétoncle noir, Chlamys varia, ont été suivies sur un cycle biologique complet, dans un marais maritime. Cette croissance s'est avérée être nettement, plus rapide qu'en rade de Brest, puisque la taille commerciale de 35 mm a été atteinte en un an. Les individus se sont reproduits au bout de la même période. Ceci montre qu'il est possible d'obtenir un cycle biologique très rapide. Lors du cycle de production, des mortalités importantes ont été constatées entre le prégrossissement en nurserie et la mise en élevage. Ces mortalités ne se sont pas renouvelées sur le cycle en cours d'étude. Un agent parasite nouveau a été décrit sur cette espèce, sans que les certitudes puissent être établies quant à son rôle pathogène.... |
| Tipo: Text |
Palavras-chave: Croissance; Composition biochimique; Elevage; Pétoncle; Marais maritime; Charente-Maritime. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00048/15905/13333.pdf |
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| Chagot, Dominique; Comps, Michel; Boulo, Viviane; Ruano, Francisco; Grizel, Henri. |
| An infection caused by a Perkinsus -like parasite has been observed in Ruditapes decussatus from Portugal (Comps and Chagot, 1987). Following a pathological study carried out on clams affected by mortalities in the Algarve area, this parasite was found again, inducing a reaction process in the host. Histological examination reveals the parasite in the connective tissue of different organs. The adjoining tissues, particularly the epithelia, are not affected. |
| Tipo: Text |
Palavras-chave: Cells; Histology; Pathology; Ruditapes decussatus. |
| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/1987/acte-3109.pdf |
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| Hervio, Dominique; Chagot, Dominique; Godin, Pierre; Grizel, Henri; Mialhe, Eric. |
| Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the subtrate (cytidine 5' monophosphate). Lead phosphate precipiation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as "dense bodies". Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostrea is discussed. |
| Tipo: Text |
Palavras-chave: Acid phosphate activity; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2755.pdf |
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| Bodoy, Alain; Garnier, Jacqueline; Geairon, Philippe; Chagot, Dominique; Grizel, Henri; Comps, Michel; Razet, Daniel. |
| Depuis le mois de juin 1988, les ostréiculteurs ont fait part de la présence de mortalités importantes sur le banc de Ronce (bassin de Marennes-Oléron), d'abord auprès de l'Ifremer et des Affaires Maritimes, puis des pouvoirs publics. Ces problèmes ont été évoqués lors d'une réunion en Préfecture de Charente-Maritime, le 4 juillet 1988. Il s'est alors avéré nécessaire de réaliser un constat de la situation notamment sur le banc de Ronce, et de faire le point sur les causes pouvant être à l'origine de la situation sur les mortalités survenues dans cette zone. Cette décision a été confirmée le 12 juillet, lors d'une réunion à la Sous-Préfecture de Rochefort. Le présent rapport présente les résultats scientifiques actuellement disponibles, ainsi que les... |
| Tipo: Text |
Palavras-chave: Mortalité; Météorolgie; Paramètres hydrologiques; Physiologie; Croissance; Bivalvia; Crassostrea gigas; Bassin de Marennes Oléron. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/00086/19765/17406.pdf |
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| Noel, Danielle; Boulo, Viviane; Chagot, Dominique; Mialhe, Eric; Paolucci, Francis; Clavies, Christine; Hervaud, Eric; Elston, Ralph. |
| Mytilus edulis develop progressive and fatal hemocytic neoplasia. We have generated monoclonal antibodies using neoplastic hemocytes from highly infected individuals collected from Puget Sound in Washington State (USA). Twenty-seven monoclonal antibodies against neoplatic cell antigens were obtained. Specificity of 2 purified antibodies, 14F1 and 16G10, was demonstrated by immunofluorescence when incubated with neoplastic hemocytes, whereas these anbidodies did not react with normal cells. This specificity was confirmed by immunogold labelling and electron microscopic visualization. The technique permitted localization of the epitopes recognized by the monoclonal antibodies on the plasma membrane. These monoclonal antibodies are suitable reagents for... |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2757.pdf |
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| Heral, Maurice; Bacher, Cedric; Razet, Daniel; Prou, Jean; Galgani, Francois; Renault, Tristan; Bodoy, Alain; Garnier, Jacqueline; Geairon, Philippe; Chagot, Dominique; Grizel, Henri; Comps, Michel; Masson, Daniel; Noinin, Michel. |
| Dès le 4 JUIN 1992, un ostréiculteur digne de confiance avait signalé aux services d'IFREMER, une situation anormale sur le banc de Ronce les Bains. Un premier prélèvement a été effectué le jour même. En effet, ce secteur avait déjà été le lieu de mortalités d'huitres estimées à 7 000 tonnes, au printemps 1988, et s'est révélé par le passé comme étant un endroit sensible. Pour information, figure en annexe le rapport établi en 1988, à destination du Préfet de la Charente Maritime. Ces mortalités ayant rapidement pris de l'ampleur, un dépôt de plainte contre X a été effectué par la Section Régionale de la Conchyliculture. A la suite de quoi, les services d'IFREMER ont été requis par la Gendarmerie Nationale (Brigade de Marennes) le 10 juin 1992, pour... |
| Tipo: Text |
Palavras-chave: Ostréiculture; Huîtres creuses; Crassostrea gigas; Mortalité; Banc de Ronce Les Bains; Bassin de Marennes Oléron. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00048/15899/13327.pdf |
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| Bachere, Evelyne; Chagot, Dominique; Tige, Gilbert; Grizel, Henri. |
| Considering the epizootics which affect the French stock of flat oysters, Ostrea edulis (Grizel, 1985), trials to introduce an Australian oyster, Ostrea angasi, have been undertaken. The spat produced in a hatchery from breeders imported from Australia were reared in the south ofBrittany and in Normandy (Bougrier et al., 1986). Following mass mortalities (65% of individuals) a haplosporidian was observed in this species. Sporogenesis of the parasite was studied using Iight and electron microscopy. |
| Tipo: Text |
Palavras-chave: Haplosporidian; Parasitology; Pathology; Ostrea angasi; Oysters. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/1986/acte-3112.pdf |
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| Lassus, Patrick; Chagot, Dominique; Miossec, Laurence; Bocquene, Gilles; Truquet, Philippe. |
| Tetrachloroethylene, a chlorinated solvent, is detected in the plume of East-Toulon domestic wastes treatment plant. Its subletal toxicity was tested du ring 31 days in a flow-through system at Pen Bron aquaculture centre, using swampshrimp Palaemonetes varians as test organism. Under expérimental conditions : natural variations in temperature and salinity , normal pH, ammonia and dissolved oxyg~n values, running tetrachloroethylene concentrations (10, 100 and 500 µg.l-1) induced neither mortalities nor any change in molting frequencies of experirnented animaIs. Histology of gills in contaminated animaIs showed several non specific deteriorations which could be observed either in control or assay animaIs. Necrosis levels nevertheless, seem to increase in... |
| Tipo: Text |
Palavras-chave: Palaemonetes varians; Crustacés; Tetrachloroethylene; Flux continu; Rejets urbains; Histologie; Nécroses; Palaemonetes varians; Crustacees; Tetrachloroethylene; Flow through system; Urban wastes; Histology; Necrosis. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00156/26707/24782.pdf |
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| Registros recuperados: 18 | |
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